과잉치 분류에 따른 치수유래줄기세포 계대 배양 시간의 연관성
Relationship with Passage Time of Human Dental Pulp Stem Cells from Supernumerary Tooth by Classification
신요섭, 김종빈, 김종수,
소속 상세정보
신요섭 ( Shin Yeo-Seob ) - 단국대학교 치과대학 소아치과학교실
김종빈 ( Kim Jong-Bin ) - 단국대학교 치과대학 소아치과학교실
김종수 ( Kim Jong-Soo ) - 단국대학교 치과대학 소아치과학교실
KMID : 0358920160430040419
Abstract
For this research 20 supernumerary teeth impacted in the maxillary anterior have been extracted and pulp cells have been collected from them. From the collected pulp cells, total of 17 (10 males, 7 females) have been selected as subjects. From this research, the run-time of successive culture of the cell from tooth number pulp tissue was 2.91 ± 0.29 days. From the gathering of cells from the initial pulp tissue until gaining 80% confluency took 4.53 ± 0.94 which was the longest. The following successive cultures took 2.73 ± 0.32 days. Average runtime for female was 2.81 ± 0.27 days whereas male had average runtime of 2.98 ± 0.29 days. Average run-time for inversion was 2.94 ± 0.30 days and for normal location, 2.80 ± 0.20 days. Average runtime was 2.92 ± 0.31 days and other forms took 2.88 ± 0.22 days. In the future, follow up research would be needed to evaluate the efficiency of the cells collected from the initial passage and the latter passage as stem-cells and taking into consideration the less than 3 days’time for the subculture, it could be concluded that the research efficiency and fast cultivation would be sufficiently effective.
키워드
과잉치 ; 줄기세포 ; 분류 ; 계대 배양
Supernumerary tooth ; Passages ; Classification ; Stem cells
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